Microglial RNA sequencing from humanized mouse model expressing schizophrenia susceptibility factor C4A

Human C4A gene locus is associated with increased schizophrenia risk. In order to investigate this genetic link, we constructed two mouse models that express human C4A or C4B, and one that overexpresses C4A. To investigate whether the isotypic effects of human-C4 gene is observed in the microglial transcriptional profile during adolescence in mice (postnatal day 40) in the medial prefrontal cortex, we conducted a bulk RNA sequencing of microglia. Our analyses showed no significant differentially expressed genes between any two groups. Microglial mRNA profiles of P40 humanized-transgenic mice containing human C4A and C4B sequences人类补体4A（C4A）基因座与精神分裂症发病风险升高存在关联。为探究该遗传关联，我们构建了两种分别表达人类C4A（C4A）或C4B（C4B）的小鼠模型，以及一种过表达C4A的小鼠模型。为验证人类C4基因的同型效应是否会在小鼠青春期（出生后第40天）的内侧前额叶皮层小胶质细胞（microglia）转录组中显现，我们对小胶质细胞开展了批量RNA测序（bulk RNA sequencing）。分析结果表明，任意两组间均未检出显著差异表达基因。携带人类C4A与C4B序列的P40天龄人源化转基因小鼠的小胶质细胞mRNA转录谱。