Transcriptome profile of tra1 mutant in S. cerevisiae

Tra1 is an essential component of both the yeast SAGA/SLIK and NuA4 complexes, where it recruits these complexes to acetylate histones at targeted promoters. Importantly, Tra1 regulates the transcriptional response to multiple stresses. This submission contains RNA sequencing data from Saccharomyces cerevisiae strains expressing a Tra1 mutant with three arginine to glutamine mutations in the PI3K domain (tra1Q3; Berg et al., 2018). Our goal was to identify how gene expression profile changes during the chronological aging process due to mutation in the PI3K domain of Tra1.Tra1是酵母SAGA/SLIK复合物与NuA4复合物的必需组成部分，其可招募这两类复合物至靶向启动子区域以介导组蛋白乙酰化。尤为关键的是，Tra1可调控细胞应对多种胁迫的转录应答。本次提交的数据集包含表达Tra1突变体的酿酒酵母（Saccharomyces cerevisiae）菌株的RNA测序（RNA sequencing）数据，该突变体的PI3K结构域（PI3K domain）内存在3处精氨酸突变为谷氨酰胺的位点（tra1Q3；Berg等，2018）。本研究的目的在于阐明Tra1的PI3K结构域发生突变后，时序衰老过程中基因表达谱的变化规律。