Decitabine induces the expression of genes in THP-1 cells

Next Generation Sequencing Facilitates Quantitative Analysis of THP-1 cells treated with DMSO and Decitabine Overall design: mRNA profiles of THP-1 cells were generated by deep sequencing, in duplicate, using Illumina Navoseq 6000. The sequence reads that passed quality filters were analyzed at the transcript isoform level with two methods: Burrows–Wheeler Aligner (BWA) followed by ANOVA (ANOVA) and TopHat followed by Cufflinks. qRT–PCR validation was performed using SYBR Green assays.