The effect of high or low fat diet on the miRNA composition of exosomes derived from adipose tissue

Exosomes are nanosized extracellular vesicles with lipid bilayer membranes and contain various contents, including lipids, miRNAs and proteins, all of which are widely involved in signaling pathways and genetic information processes. Exosomes derived from adipose tissues (AT-Exos) have been identified as a crucialmedium in the transmission of information from adipose tissue to itself and to other organs, including exosomes derived from inguinal white adipose tissue (iWAT-Exos), visceral white adipose tissue (vWAT-Exos) and brown adipose tissue (BAT-Exos). Here we reported that dietary conditions and tissue origins of exosome can affect the composition and function of miRNAs in AT-Exos, mainly including lipid metabolism and inflammatory signaling pathways associated with metabolic imbalance. To describe the miRNA profiles of AT-Exos under diet-induced obesity, we isolated WAT-Exos and BAT-Exos from 6 weeks wild-type C57BL/6 (B6) mice fed a high-fat diet (HFD) or low-fat diet (LFD) over 4 months. We then performed miRNA profiling analysis using data obtain from miRNA-seq of 4 different AT-Exos. Comparative miRNA profiling analysis of miRNA-seq data for LFD-WAT-Exos and HFD-WAT-Exos, LFD-BAT-Exos and HFD-BAT-Exos, LFD-WAT-Exos and LFD-BAT-Exos, HFD-WAT-Exos and HFD-BAT-Exos.