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Structure–function mapping of a heptameric module in the nuclear pore complex

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https://www.omicsdi.org/dataset/bioimages/S-JCBD-201109008
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Localization of a Nup49p-CFP reporter in the presence of the indicated Nup variant as determined by fluorescence microscopy. Cells were visualized at room temperature with a 63x, 1.4 numerical aperture plan-apochromat objective (Zeiss) using a Carl Zeiss Axioplan 2 microscope equipped with a Hamamatsu Orca ER-cooled CCD camera. The system was controlled with Openlab imaging software (Perkin Elmer). Final images were assembled, and gamma levels adjusted to enhance contrast only, using Adobe Photoshop software.
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2018-11-29
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