Table S1 - Production of scFv-Conjugated Affinity Silk Powder by Transgenic Silkworm Technology

In all primers, lower-case letters indicate restriction sites for EcoRI (gaattc), BamHI (ggatcc), HindIII (aagctt), BglII (agatct), and NotI (gcggccgc). cDNA fragments for the fibroin L-chain promoter region through the fibroin L-chain coding region (FibLpro–FibL) and the fibroin L-chain 3′-untranslated region (FibL-3′-UTR) were generated by PCR from pBac (3xP3-DsRed2+L-chain-GFP) [13] with the following primer sets (FibLpro–FibL: sense primer #1 and reverse primer #2, FibL-3′-UTR: sense primer #3 and reverse primer #4). A cDNA fragment for anti-WASP-scFv-Myc was generated by PCR from pCAG/anti-WASP-21HL [20] using sense primer #5 and reverse primer #6. These PCR products were digested with EcoRI-BamHI, HindIII-BglII, and BamHI-HindIII, respectively, and cloned together into the EcoRI-BglII site of the pBac[3xP3-DsRed2afm] vector. This construct was designated pBac[3xP3-DsRed2afm]-LLL-anti-WASP-scFv-Myc. The control plasmid vector, pBac[3xP3-DsRed2afm]-LLL-EGFP-His, was modified by the insertion of a 6×His tag sequence at the C-terminal coding region of fibroin L-chain and EGFP fusion protein in the original plasmid construct [13]. cDNA fragments for mouse WASP exons 1–5 (aa 1–171, designated WASP15) and exons 6–9 (aa 172–313, designated WASP69) were generated by PCR from mRNA of C57BL/6 mouse spleen with the following primers: WASP15, sense primer #7 and reverse primer #8; WASP69, sense primer #9 and reverse primer #10. These PCR products were digested with NotI, and cloned into the pGEX-4T-2 expression vector. (DOC)