Gene expression in three independent resynthesized Brassica napus allopolyploid lines and their diploid progenitors

We anlalyzed gene expession using an Arabidopsis 26K 70mer spotted microarray Differential expression was tested using two ANOVA models: 1) a per-gene variance ANOVA, and 2) a common variance ANOVA We compared gene expession between the diploid progenitors (Brassica rapa and Brassica oleracea), and tested mid-parent expression in the allopolyploids by comparison to a reference sample (composed of a 1:1 mix of parental RNA). Two biological replicates (composed of pooled leaf tissue) were each analzed with two dye swaps in a randomized complete block design(RCBD).