Tajima’s test for neutrality.

Statistical analysis was carried out using MEGA5. In the statistical analysis, all the positions with site coverage 95% site coverage were eliminated. That is, fewer than 5% alignment gaps, missing data, and ambiguous bases were allowed at any position. All ambiguous positions were removed for each sequence pair. Abbreviations: m = number of sequences; S = number of segregating sites; Ps = S/n; Θ = ps/a1; π = nucleotide diversity; and D is the Tajima test statistic.