Uncovering small non-coding RNAs involved in carcinogenesis of Glioma

To determine which signalling pathways are affected by small RNAs (piRNAs, miRNAs) through target regulation in Glioblastoma Multiforme (GBM), we performed high-throughput next-generation sequencing in U87-MG GBM cell line. The RNA sequencing (RNA-Seq) of small RNAs and transcriptomes discovered both known and novel piRNAs and miRNAs as well other transcriptomes of protein-coding genes, lncRNAs and pseudogenes expressed in this GBM cell line. These small RNAs and target transcriptomes can be further investigated to decode novel molecular mechanisms underlying oncogenesis of this malignancy. Overall design: Small RNA and transcriptome profiles of glioblastoma cell line were generated by Illumina HiSeq 2500 platform. After single-end sequencing of small RNAs and pair-end sequencing of transcriptomes, the adapter was trimmed, and filtered reads analyzed to discover the known and novel small RNAs and mRNAs, which might be involved in tumorigenesis of glioblastoma.