Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays. Screening for potential target genes of hsa-mir-199a-1 and hsa-mir-4423 using Clariom D arrays

We screened for target genes of hsa-mir-199a-1 and hsa-mir-4423 by transcriptome profiling. The miRNAs were overexpressed in HeLa cells by transient transfection of precursor miRNA expression plasmids. High-density Clariom D arrays for human were used for the gene expression analysis. This GEO entry provides the microarray data preprocessed according to the gene level workflow. Overall design: HeLa cells were transiently transfected with non-viral precursor miRNA expression plasmids from GeneCopoeia (HmiR0296-MR04 and HmiR1083-MR04, respectively). As negative control, a plasmid with scrambled sequence (CmiR0001-MR04, GeneCopoeia) was used. Total RNA was isolated 24 h (n=2 replicates) and 48 h (n=4 replicates) following transfection. For all 18 RNA samples, transcript levels were measured with Clariom D arrays.