Four cultivars of Mandarin, i.e., Nagpur Mandarin, Khasi Mandarin, Sikkim Mandarin, and Coorg Mandarin, and two cultivars of acid lime, i.e., NRCC7, and NRCC8, were resequenced. The study aims to do a comparative study with the other citrus genomes already available in the public database and to study the genome evolution of Indian cultivars. The genome data is also used to identify SNPs with peculiar characteristics like taste and flowering more than once a year.

Reference genome sequences of several citrus species are now available, and this information permits both the rapid identification of candidate genes through bioinformatics analysis and single nucleotide polymorphism SNP discovery through comparison of the reference with sequence data from different cultivars. The association of markers with heritable traits is used to associate the genotype of an organism with the expressed phenotype. These markers can be used routinely in citrus breeding programs, for their improvement, genetic diversity analysis, cultivar identification, phylogenetic analysis, characterization of genetic resources, and association with agronomic traits. Four cultivars of Mandarin?Nagpur Mandarin, Khasi Mandarin, Sikkim Mandarin, and Coorg Mandarin?were resequenced. Nagpur Mandarin is chiefly grown in Satpura hills, Vidarbha region of Central India. Khasi and Sikkim Mandarins grow in the Northeastern regions, and Coorg Mandarins grow in the Southern regions.