GNPS - Untargeted Metabolomic Profiling of Streptomyces and Nocardia from Oligotrophic Ecosystems

We conducted an untargeted metabolomic analysis of Streptomyces and Nocardia strains isolated from two contrasting oligotrophic environments: Cuatro Cienegas and Calakmul. Metabolomic profiling was performed using liquid chromatography coupled to high-resolution tandem mass spectrometry (LC-HR-MS/MS). Data acquisition was carried out in positive ionization mode under a Data-Dependent Acquisition (DDA) strategy. The analysis included samples cultivated under two distinct culture conditions: ISP2 and ISP5 media. Metabolites were extracted using a solvent mixture of acetonitrile, methanol, and ethyl acetate in a 1:1:1 ratio