Leaf endomicrobiomes of early and late planted sorghum seedlings

Leaf fungal and bacterial communities from field-grown sorghum accessions (KS and SD) were characterized in response to chilling and non-chilling temperatures using amplicon sequencing. Bacterial primers 799F and 1115R were used to amplify 16S V5-V6. Fungal primers ITS1f and ITS2 were used to amplify ITS1. Locus-specific PCR was followed by index PCR with Illumina Nextera Kit v2 sets A, B, C, D. After PCR clean-up, amplicons were pooled equimolarly and sequenced on an Illumina MiSeq 2 x 300 at South Dakota State University Genomics Sequencing Center.

对田间种植的玉米（KS和SD）品系中的叶部真菌和细菌群落，通过扩增子测序技术进行了表征，以探讨其在冷害与非冷害温度条件下的响应。本研究采用细菌引物799F和1115R进行16S V5-V6区域的扩增，真菌引物ITS1f和ITS2用于ITS1区域的扩增。随后，通过特定位点PCR扩增，并使用Illumina Nextera Kit v2套件A、B、C、D进行索引PCR。PCR产物纯化后，等摩尔混合的扩增子序列在南达科他州立大学基因组测序中心采用Illumina MiSeq 2 x 300进行测序。