A Transcriptomic Dataset of Liver Tissues from Global and Liver-Specific Bmal1 Knockout Mice

The circadian clock system regulates a wide range of physiological processes in mammals, and the core circadian clock gene Bmal1 is crucial for maintaining the oscillations of circadian clock system by controlling the rhythmic expression of numerous clock-controlled genes. These data offer a valuable resource for researchers studying the role of BMAL1 in liver physiology and pathology, as well as the broader field of circadian biology. To explore the transcriptional changes associated with Bmal1 deletion in liver tissues, we collected the liver tissues of global and liver-specific Bmal1 knockout mice at two circadian time points (CT2 and CT14), and used them for RNA-seq analysis. Bmal1+/mice on C57BL/6J background were bred to generate Bmal1+/+ and Bmal1-/- mice . The Bmal1f/f (F) and Alb-Cre mice were bred to generate L-Bmal1/ (liver- specific Bmal1 knockout, LKO) mice. KO = global Bmal1 knockout LKO = liver-specific Bmal1 knockout F (Bmal1f/f) = the control mice for LKO (liver-specific Bmal1 knockout, Alb-Cre; Bmal1f/f ); These mice (Bmal1f/f) have a floxed Bmal1 genotype but do not express Cre, serving as appropriate controls for the liver-specific knockout.昼夜节律钟系统调控哺乳动物体内诸多生理过程，核心昼夜节律钟基因Bmal1通过控制大量时钟调控基因的节律性表达，对维持昼夜节律钟系统的振荡至关重要。本数据集为研究Bmal1在肝脏生理与病理过程中的作用，以及更广范围的昼夜节律生物学领域的科研人员提供了宝贵的研究资源。 为探究肝脏组织中Bmal1敲除相关的转录组变化，本研究收集了全局Bmal1敲除与肝脏特异性Bmal1敲除小鼠在两个昼夜节律时间点（CT2与CT14）的肝脏组织，并进行了RNA测序（RNA-seq）分析。 在C57BL/6J遗传背景下的Bmal1+/-小鼠经繁育，获得Bmal1+/+与Bmal1-/-小鼠。将Bmal1f/f（简写为F）小鼠与Alb-Cre小鼠繁育，得到L-Bmal1/（肝脏特异性Bmal1敲除，即LKO）小鼠。 本研究中缩写定义如下： KO = 全局Bmal1敲除； LKO = 肝脏特异性Bmal1敲除； F（Bmal1f/f）= LKO的对照小鼠，即Alb-Cre; Bmal1f/f；此类小鼠的Bmal1基因携带floxed基因型，但不表达Cre重组酶，可作为肝脏特异性敲除实验的合适对照。