A Coordinated Proteomic Approach for Identifying Proteins that Interact with the E. coli Ribosomal Protein S12.

Data from PASSEL: [[https://db.systemsbiology.net/sbeams/cgi/PeptideAtlas/PASS_View?datasetPassword=IR4769n&identifier=PASS00136 PASS00136]]. Experiment: 1st exerimental NAc pQ MeS, data file: F571953.mgf. Data published as part of J Proteome Res. 2013 12:1289-99 . From the Abstract: {{i}} ... To follow-up on this study, the D88 containing loop was probed to identify candidate binders employing a two-step complementary affinity purification strategy. The first involved an endogenously expressed S12 protein containing a C-terminal tag for capturing S12 binding partners. The second strategy utilized a synthetic biotinylated peptide representing the D88 conserved loop region for capturing S12 loop interaction partners. Captured proteins from both approaches were detected by utilizing SDS-PAGE and one-dimensional liquid chromatography-tandem mass spectrometry. ...{{/i}}