Functional inhibition of core spliceosomal machinery activates intronic premature cleavage and polyadenylation of pre-mRNAs

To understand if U1/U6/U4/U2 snRNPs could regulate 3'-UTR APA, we treated NIH3T3 cells with different concentration of U1/U6/U4/U2 AMOs Overall design: Total RNAs were harvested at 12 hour time points upon U1/U6/U4/U2 AMOs transfection,mRNA 3'-seq library was constructed using lexogen kit (REV. 016.24). Bioinformatics analysis were subsequently carried out using our published pipeline QuantifyPoly(A) (https://pubmed.ncbi.nlm.nih.gov/34255024/)