REFERENCE DATASET: Expression of 97 surface markers and 462 mRNAs in 49057 cells from healthy young and healthy old bone marrow

Seurat v3 object

ASSAYS:

AB: Antibody expression data

RNA: mRNA expression data

BOTH: mRNA and Antibody expression data, concatenated

integrated: Antibody and mRNA expression matrices were concatenated and data was integrated across patients using scanorama

DIMENSIONALITY REDUCTION

MOFAUMAP: UMAP performed on MOFA dimensions. Display used in the manuscript. Set reduction="MOFAUMAP"in most cases!

Projected

MOFA: Multi-OMICS factor analysis to integrate AB and RNA data. MOFA was run on scanorama-integrated data and served as input for clustering and further dimensionality reduction.

MOFATSNE: UMAP performed on MOFA dimensions.

METADATA

Batch: Source individual (BM1-3, Aged1-3).

class: Type of individual (healthy young, healthy old).

Change log: 

v3: Compared to the previous version of this file, the 3 AML patients were removed. A new object with 15 AML patients has been created: https://doi.org/10.6084/m9.figshare.14780127.v1

v4: Objects were slimmed to correspond to the information described in our study. Data now only contains relevant dimensions reductions and metadata columns; unused RNA and antibody targets were excluded from the objects.