Ferroxidase assay data on Rru_EncFtn and Rru_Enc:EncFtn proteins

1st and 2nd Excel tabs:Ferroxidase activity of Rru_A0973 (with or without Zn(II) and A0974/A0973 encapsulated ferritins.Ferroxidase assays (average, standard deviation, n >=3) performed on on Rru_A0974/A0973 (wild-type and E31A, E34A, W38A variants) and Rru_A0973 (wild-type and E31A, E34A, W38A, E31A/E34A) proteins in the presence of 50 uM Fe(II).A0973 concentration was kept at 10 uM in both data sets.Protein sample was incubated with 50 μM FeSO4.7H2O (10 times molar equivalent Fe(II) per FOC) and progress curves of the oxidation of Fe(II) to Fe(III) was monitored at 315 nm at room temperature. The background oxidation of iron at 50 μM in enzyme-free control is shown for reference (dotted grey line). Solid lines represent the average (n = 3) of technical replicates, shaded areas represent standard deviation from the mean. Protein and iron samples were prepared anaerobically in Buffer H (10 mM HEPES pH 8.0, 150 mM NaCl), and 0.1 % (v/v) HCl, respectively.3rd Excel tab:Linear Regression on first 200s of ferroxidase assays with Rru_A0973 and Rru_A0974/A0973. Data from first 200 s of the assays described above were processed using the Linear Regression tool in GraphPad (Prism8) to determine v0, initial enzymatic rate, from slope of each of the linear curves. Variants initial enzymatic rate (v0variant) calculated from linear regression curves in the first 200 s of ferroxidase assay experiments were divided by the slope calculated for the corresponding wild-type species (v0wild-type). 4th Excel tabLinear Regression on first 10 s of ferroxidase assays with A0973_E31A and A0974/A0973_E31A.Data from first 10 s of the assays carried out with 10 μM Rru_A0973_E31A or Rru_A0974/A0973_E31A were processed using the Linear Regression tool in GraphPad (Prism8) to determine v0, initial enzymatic rate, from slope of each of the linear curves.