Cornulin transcriptomic analysis in Cal27 oral cancer cells

RNA-Seq profiling of Cal27 Oral squamous carcinoma cells overexpressing Cornulin (CRNN), GFP control, and untreated group (UNT) to study differential gene expression. This project investigates the transcriptomic alterations induced by the overexpression of Cornulin (CRNN) in the Cal27 human oral squamous cell carcinoma cell line. The study design included three experimental groups: (1) Cornulin-overexpressing Cal27 cells, (2) GFP-expressing Cal27 cells as vector controls, and (3) untreated Cal27 cells. Total RNA was extracted using Trizol reagent, followed by RNA quality assessment via Bioanalyzer. Strand-specific mRNA libraries were prepared using the TruSeq Stranded mRNA kit, pooled at 6 nM, and sequenced using an Illumina NovaSeq 6000 S4 flow cell in a paired-end (2x100 bp) configuration. Raw FASTQ files were analyzed by aligning to the human genome (GRCh38), and gene-level quantification was performed. Differential expression analysis was conducted using the DESeq2 R package. DEG lists from each comparison group were subjected to pre-ranked gene set enrichment analysis (GSEA) to identify enriched pathways and gene ontologies. The dataset provides insights into Cornulin-mediated gene regulation. Overall design: Paired-end stranded RNA sequencing of Cornulin-overexpressing Cal27 cells vs. GFP vector control and untreated Cal27 cells. Two biological replicates per group.