Notch1 dose modulates endothelial gene expression

Purpose: To use RNA-Seq analysis of endothelial cell in various Notch1 alllels in order to determine transcrptional differences as a consequence of Notch dose. Methods: Using a FACS sorting we generated high-throughput RNA-SEQ data of endothelials in various Notch1 alleles during development Results: Notch1 dose can alter gene expression in a subset of endothelial genes RNA-Seq was performed on endothelial cells isolated at e9.5 from embryos with various Notch1 alleles including N1+/+, N1+/-, N1+/vg, N112/vg, N112/-研究目的：通过对携带不同Notch1等位基因的内皮细胞进行RNA测序（RNA-Seq）分析，探究Notch1剂量变化所导致的转录差异。实验方法：采用荧光激活细胞分选术（Fluorescence-Activated Cell Sorting, FACS），我们针对发育过程中不同Notch1等位基因背景下的内皮细胞生成了高通量RNA测序数据。实验结果：Notch1剂量可改变部分内皮基因的表达模式。本次研究对胚胎发育第9.5天（e9.5）分离得到的内皮细胞开展了RNA测序，所用胚胎携带多种Notch1等位基因，包括N1+/+、N1+/-、N1+/vg、N112/vg及N112/-。