Mutational data for protein stability

FireProtDB is a comprehensive, manually curated database of the protein stability data for single-point mutants. Proteins find their use in numerous biomedical and biotechnological applications. Naturally occurring proteins usually cannot withstand harsh industrial environments since they have evolved to function under mild conditions. Increasing protein stability is one of the key determinants of protein applicability. The predictive power of the current computational tools is compromised by the limited experimental data that would allow a rigorous training and testing. This database combines the published datasets from ProTherm and ProtaBank, the data extracted from the recent literature, and the measurements collected in our laboratory. The annotations were obtained from VariBench and HotSpot Wizard. The graphical user interface is designed to facilitate both types of the expected use: (i) the interactive explorations of individual entries on the level of a protein or a mutation and (ii) the construction of highly customized, machine learning-friendly datasets using advanced searching and filtering.FireProtDB是一款经人工手动整理甄选的综合性数据库，收录单点突变体（single-point mutants）的蛋白质稳定性数据。蛋白质被广泛应用于众多生物医学与生物技术领域。天然蛋白质通常无法耐受严苛的工业环境，这是由于其在演化过程中仅适配温和的生理条件。提升蛋白质稳定性是决定其应用可行性的核心要素之一。当前的计算工具因可用实验数据匮乏，难以开展严谨的模型训练与测试工作，因此其预测能力受到限制。该数据库整合了来自ProTherm与ProtaBank的公开数据集、从近期学术文献中提取的数据，以及本实验室采集的实测数据。其注释信息源自VariBench与HotSpot Wizard。该数据库配备的图形用户界面（Graphical User Interface）旨在支持两类预期使用场景：其一，可在蛋白质或突变层面实现单条数据条目的交互式探索；其二，可通过高级搜索与过滤功能，构建高度定制化、适配机器学习的数据集。

搜集汇总

背景与挑战

背景概述

该数据集是一个全面且经过人工整理的蛋白质稳定性突变数据库，专注于单点突变数据。它整合了多个公开数据集和实验室测量数据，旨在支持交互式探索和机器学习友好型数据集的构建。