RNA-Seq of 120 hpf control sibling and mutant uhrf1 hi272 zebrafish livers

In this study, we performed RNA-seq on 5 dpf livers of uhrf1-hi272 mutants and phenotypically wild-type siblings collected at 120 hpf. "To perform RNA-seq on pools of uhrf1-hi272 mutant and sibling livers at 5 dpf, we collected between 15-30 livers from 5 independent clutches of uhrf1-hi272 mutants and their phenotypically WT siblings which were sorted based on phenotype following immobilization using tricaine. We extracted total RNA from livers to generate libraries as per TRIzol protocol (https://www.thermofisher.com/ae/en/home/references/protocols/nucleic-acid-purification-and-analysis/mrna-protocols/trizol-plus-rna-purification-kit.html#prot2). Total RNA was DNAseI treated, depleted for ribosomal RNA by using RiboZero and used for Illumina Library prep. RNA was analyzed on an Agilent 2100 Bioanalyzer. Illumina TruSeq RNA sample preparation version 2 protocol with Ribo-Zero Gold. cDNA libraries were sequenced on the Illumina NextSeq500 platform to obtain 75 bp single-end reads.