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The estimated ABCH2 pmoles in our preparation (S7 Fig) allows us to extract the pmoles of Pi per pmole of ABCH2 per minute of reaction.

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As shown in red, the hydrolysis rate is ~178 pmoles of Pi/ pmole of ABCH2/ minute of reaction. Even if our estimation is indirect and prone to errors, the rate value is substantial indicating that ABCH2 undergoes consecutive ATP hydrolysis rounds. (XLSX)
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2023-08-16
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