Quantification of HSV protein positive neurons and association with an inflammatory cuff post-hyperthermic stress of C57BL/6 mice latently infected with McKrae or 17syn+.

Mice latently infected with McKrae or 17syn+ were subjected to hyperthermic stress and ganglia were harvested at the indicated times post-hyperthermic stress (phs) (n = 5–7 per experiment; results pooled from 2 independent experiments at each time point). HSV proteins were first detected in the whole ganglia and TG were subsequently embedded, cut as 10 μm sections, and counterstained with cresyl violet, as described in Methods. Each HSV protein positive neuron in the sectioned material was then related back to the whole ganglia tissue analysis to confirm the identity as “intact” or “fragmented” and assessed for association or lack of association with an inflammatory cuff, determined as ≥4 inflammatory cell radius (see Fig 6).