Raw qPCR data

(A) Expression of the new TlicBgluc gene was evaluated by qPCR at different stages of insect life (egg, larval stage early –ELI, late larval stage - LLI, pupa, moth) to determine the stage at which the gene exhibits higher expression. According to the results, the gene was widely expressed throughout the insect's life cycle, but expression increased significantly at the late larval stage (LLI), when the insect required most glucose utilization. (B) In the larval stage, TlicBgluc expression was evaluated in the midgut and carcass. Gene transcripts are massively detected in the gut. TlicGAPDH and TlicRPS18 were chosen as endogenous normalizing genes, and relative expression was calculated using the 2-ΔCt method. Bars represent 5% standard error.