<i>in silico</i> off-target analysis of lethal genes in cabbage stem flea beetle

Off-target analysis was conducted using Bowtie v1.3 (Langmead et al., 2009) to map every possible 21-mers of CSFB effective lethal gene sequences. Mappings in both directions were allowed with up to 2 mismatches. Furthermore, the orthology inference analysis described above was conducted using translated amino acid sequences of <i>T.castaneum</i> and the transcriptomes of the investigated off-target organisms Aphis mellifera (RefSeq: GCF_003254395.2), Bombus Terrestris (RefSeq: GCF_910591885.1), Daphnia magna (RefSeq: GCF_020631705.1), <i>Coccinella septempunctata </i>(RefSeq: GCF_907165205.1), and Chrysoperla carnea (RefSeq: GCF_905475395.1). The orthology inference results were filtered to get the single orthologs of the lethal genes associated with above 90% mortality in (Buer et al., 2024) The filtered list and the off-target prediction results were investigated to determine the number of matches to the putative lethal gene orthologs in the off-target organisms. "off_target_prediction_lethal" is the Python 3.9 script used during data analysis.