Intracellular content of trehalose following different stress treatments in exponential phase cultures of the parental strain (WT) and its congenic mutant deficient in acid trehalase (KO).

Yeast cells were grown at 30°C in YPD until they reached exponential phase (OD600  =  1.0–1.2). The samples were prepared and the trehalose content was measured as described in Methods. The results are the mean ± SD of one representative experiment of two performed in triplicate. The distinction between the treated samples and control values obtained was significant at **P<0.01 and ***P<0.001 according to the Student t-test.