Human Protein Atlas - Cell Atlas

The Cell Atlas provides high-resolution insights into the expression and spatio-temporal distribution of proteins within human cells. Using a panel of 64 cell lines to represent various cell populations in different organs and tissues of the human body, the mRNA expression of all human genes are characterized by deep RNA-sequencing. The subcellular distribution of each protein is investigated in a subset of cell lines selected based on corresponding gene expression. The protein localization data is derived from antibody-based profiling by immunofluorescence confocal microscopy, and classified into 32 different organelles and fine subcellular structures. The Cell Atlas currently covers 12390 genes (63%) for which there are available antibodies. It offers a database for exploring details of individual genes and proteins of interest, as well as systematically analyzing transcriptomes and proteomes in broader contexts, in order to increase our understanding of human cells.

细胞图谱项目提供了关于人类细胞内蛋白质表达及其空间-时间分布的高分辨率见解。该项目采用64个细胞系，代表人体不同器官和组织中的多种细胞群体，通过对所有人类基因的mRNA表达进行深度RNA测序进行特征描述。基于相应的基因表达，选取细胞系子集研究每种蛋白质的亚细胞分布。蛋白质定位数据通过基于抗体的免疫荧光共聚焦显微镜分析获得，并归类为32种不同的细胞器和精细的亚细胞结构。目前，细胞图谱涵盖了12390个基因（占比63%），对于这些基因均有可用的抗体。它提供了一个数据库，用于探索感兴趣的个体基因和蛋白质的详细信息，并可在更广泛的背景下系统性地分析转录组和蛋白质组，以增进我们对人类细胞的理解。