Expression data from glioblastoma surgical specimens

Surgical resection is the first choice of the standardized treatment scheme for glioblastoma and is performed under general anesthesia. Previous researches have shown that propofol and sevoflurane have different effects on the biological process of glioma cells from the molecular level. Some of them, for instance, control the glioma cell proliferation, invasion and migration In vitro or In vivo. We used mRNA microarray to discuss the effects of propofol and sevoflurane on gene expression in patients with glioblastoma. The correlation between DEGs and patients’ prognosis was further analyzed. Glioblastoma specimens were obtained from neurosurgery under aseptic conditions for RNA extraction and hybridization on Affymetrix microarrays. We sought to obtain the change of glioblastoma prognostic-related genes under the treatment of propofol and sevoflurane respectively. To that end, we divided the patients into TIVA group (received propofol-remifentanil intraoperatively) and INHA group (received sevoflurane-remifentanil intraoperatively). The specimens used for gene microarray analysis were matched according to the time of anesthesia and operation, tumor location and grade, and pathological type (IDH, Ki-67, etc.).