Regional differences in neonatal enterocytes

We report bulk scRNAseq data obtained from 2 P6 mice. Input was isolated epithelium cells of the small intestine in a 2cm segment of the jejunum and 2 cm segment in the ileum of the same mouse, 1 male and 1 female were included in the analysis. A 2 cm segment of jejunum and ileum was isolated from the same mouse. The segments were incubated in EDTA to separate intestinal epthelial cells from the intestinal wall and then incubated in dispase to generate a single cell suspension. Cells were barcoded using PIP-Seq technology.