Bap1 loss leads to decreased expression of lineage specific commitment genes, and increased expression of pluripotency genes [RNA-seq]

We performed morpholino-mediated knockdown of Bap1 protein expression in Xenopus laevis developing embryos, and analyzed gene expression at stage 12. We find that inhibition of Bap1 leads to decrease in lineage specific commitment genes, and increased expression of pluripotency genes. RNA profiles of stage 12 embryos injected with either Ctrl morpholino (CtrlMO) or mopholino targeting Bap1 (Bap1MO), in triplicate, using Illumina Nextseq500.