Effects of cold stress changes on subchondral bone and cartilage of tibial knee joint in rats and molecule related to hypothermia and arthritis. Effects of cold stress changes on subchondral bone and cartilage of tibial knee joint in rats and molecule related to hypothermia and arthritis

Forty eight-week-old male SD-rats were randomly divided into four groups: control group (4 weeks), hypothermia group (4 weeks), control group (8 weeks) and hypothermia group (8 weeks). The hypothermia group was fed at 4±2℃, and the normal group was fed at 25±2℃. After 4 weeks and 8 weeks, Micro-CT and saffron solid green staining were performed to observe the conditions of subchondral bone and cartilage. At 4 weeks, rat subchondral bone tissue was cut for second generation high-throughput sequencing, differential gene screening and gene enrichment analysis, and PCR validation. Overall design: To find out which molecules play a role in hypothermia and arthritis, we performed high-throughput sequencing of subchondral bone in rats at low and normal temperatures, followed by PCR validation