Dynamic upregulation of retinoic acid RA signal in the early postnatal murine heart promotes cardiomyocyte cell cycle exit and maturation [PRVCMs_Cont-ATRA]

Retinoic acid (RA), the major active metabolite of vitamin A, is essential for organogenesis, homeostasis, and tissue morphogenesis. Despite the importance of RA signaling in embryo heart development, little is known about its function in the early postnatal period. For a comprehensive transcriptional evaluation of the effect of RA signaling on neonatal cardiomyocyte cell cycle arrest and maturation, we performed RNA-seq analysis of cultured perinatal cardiomyocytes treated with all-trans retinoic acid (ATRA) in vitro. Overall design: Comparative gene expression profiling analysis of RNA-seq data for culture prenatal rat ventricular cardiomyocytes (PRVCMs) treated with 10 µM ATRA or Dimethyl sulfoxide (DMSO) as vehicle for 48 h.