Chromatin remodeling events associated with gARID are essential for gametocyte development in Plasmodium [ChIP-seq]

To investigate the genome-wide binding sites of gARID in gametocytes of Plasmodium berghei, the chromatin immunoprecipitation followed by high-throughput sequencing (ChIP-seq) analyses were performed. Parasites expressing GFP-fused gARID were harvested after treating the infected mice with sulfadiazine and subjected to ChIP-seq experiments using anti-GFP antibody. Binding sites of gARID were determined from the sequence data. Overall design: Mice were infected with transgenic parasites and treated with sulfadiazine in their drinking water. Chromatins associated with GFP-tagged gARID were IPed with anti-GFP antibody, and DNA fragments purified from the IPed chromoatins were sequenced by the Next Generation Sequencing. Using non-IPed samples as a control (INPUT), binding sites of gARID were determined from the sequence data.