Gene expression profiles of Fir1 (RNAi) animals. Schmidtea mediterranea

The goal of this study was to identify Fir1 downstream genes that were required for local proliferation. Fir1(RNAi) animals displayed reduced local proliferation near the wound,while the proliferation far from the wound was not decreased after Fir1 RNAi. The regenerating pieces following Fir1 RNAi were expected to only show decreased levels of the genes near the wound relative to control RNAi animals. Overall design: Animals were administrated by Fir1 dsRNA for 8 days, amputated after pharynx. 48 hours later, RNA from the region adjacent to the wound(part1) and the region far from the wound(part2) in Fir1(RNAi) tail pieces was harvested with Trizol. Two biological replicates were used. Then the RNA was hybridized on a microarray with RNA from control RNAi animals.