Cell Reports; Nychyk et al 2021; Figure 7; Protein quality and quantity influence the effect of dietary fat on weight gain and tissue partitioning via host-microbiota changes

This experiment was to done to analyze the impact of dietary macronutrients and the associated microbiome on body weight. We used 2 different diets and one mouse strain (C57BL/6).
This file contains the individual data of male C57BL/6 mice exposed to 12 weeks feeding on one of 2 different diets. Body weight recorded every 3-4 days for each mouse; final body weight gain calculated at the end of each treatment period. 9 to 10 animals were exposed to each diet. Dietary treatments consisted of one level of dietary fat (55% by energy) combined with one level of protein (30% by energy) with either casein (CAS) or whey protein isolate (WPI). Full details of the dietary compositions can be found in the supplementary table 1. First, animals were fed for 4 weeks with either 30% CAS or WPI in a 55% fat diet and switched to a 4-week antibiotic (ABX) treatment (i.e., CAS-ABX, WPI-ABX) or they continued to receive the same diet (CAS-control, WPI-control). For the final 4 weeks of the experiment, the two control groups acted as faecal donors for the two groups which had received antibiotics. Complete meta-data are in the meta-data tab of the file. Means for each diet derived from these individual values are presented in Figure 7B and C.

本实验旨在分析膳食宏量营养素及其相关微生物群对体重的影响。研究采用了两种不同的饮食和一种小鼠品系（C57BL/6）。该文件包含了暴露于两种不同饮食中的一种，持续12周饲养的雄性C57BL/6小鼠的个体数据。每3至4天记录每只小鼠的体重；在每个治疗周期结束时计算最终体重增加。每个饮食组均暴露于9至10只动物。膳食处理包括一种膳食脂肪水平（占能量55%）与一种蛋白质水平（占能量30%）的结合，分别使用酪蛋白（CAS）或乳清蛋白分离物（WPI）。膳食组成的详细信息可在补充表1中找到。首先，动物在4周内以30% CAS或WPI在55%脂肪的饮食中饲养，然后切换至4周的抗生素（ABX）治疗（即CAS-ABX，WPI-ABX），或者继续接受相同的饮食（CAS对照，WPI对照）。在实验的最后4周，两个对照组作为粪便捐赠者，为接受抗生素治疗的两组提供粪便。完整的元数据可在文件的元数据标签页中找到。源自这些个体值的每种饮食的平均值在图7B和C中展示。