Chronic hypoxic response for human bronchial epithelial culture [bulkRNA-Seq batch2]

Purpose: To identify the gene expression change under chronic hypoxia on HBE cells. Methods: bulk RNA-seq was performed on primary human bronchial epithelial cells (HBE) that were cultured on air-liquid interface (ALI) condition and treated under normoxia or hypoxia (1% O2) for 5days. Results: inflammatory cytokines, collagen degradation, and angiogenic genes were upregulated under chronic hypoxia. HBE cells from six different donors were cultured and mRNA was harvested for the analysis of mRNA profile.