a comprehensive database of exosome molecular biomarkers and disease-gene associations

Exosomes play a crucial role in intercellular communication and can be used as biomarkers for diagnostic and therapeutic clinical applications. However, systematic studies in cancer-associated exosomal remain a big challenge. Here, we developed ExMdb, a comprehensive database of exosome molecular biomarkers and disease-gene associations curated from published literature and high-throughput datasets. ExMdb contains 4,586 experimentally supported gene-disease associations and 13,768 diagnostic and therapeutic biomarkers identified from experimental validation. By using ExMdb, users can quickly query detailed information onexosome features and sub-cellular locations for 28,314 genes manually curated from the literature and related data sources and 172 high-throughput datasets, including bulk and single-cell RNA sequencing (scRNA-seq) data. ExMdb also provides several user-friendly flexible tools to retrieve and analyse personalized immune infiltration, gene expression, cell cluster distribution, cell development trajectories, functional enrichment, and all possible related interactions of exosome features. Collectively, ExMdb will serve as an essential resource for investigating the regulation mechanism of complex diseases and improving the development of diagnostic and therapeutic biomarkers.The ExMdb is available athttp://www.bio-server.cn/ExMdb.

外泌体（Exosomes）在细胞间通讯中发挥关键作用，可作为临床诊断与治疗应用的生物标志物（biomarkers）。然而，针对癌症相关外泌体的系统性研究仍面临巨大挑战。本研究开发了ExMdb——一个从已发表文献与高通量数据集中整理得到的外泌体分子生物标志物与疾病-基因关联综合数据库。ExMdb包含4586个经实验验证支持的基因-疾病关联条目，以及13768个经实验确认的诊断与治疗用生物标志物。用户可通过ExMdb快速查询从文献、相关数据源以及172个高通量数据集中手动整理得到的28314个基因的外泌体特征与亚细胞定位详细信息，这些高通量数据集涵盖批量RNA测序与单细胞RNA测序（scRNA-seq）数据。ExMdb还提供多款便捷灵活的工具，用于检索与分析个性化免疫浸润、基因表达、细胞簇分布、细胞发育轨迹、功能富集情况，以及外泌体特征的所有潜在相关相互作用。综上，ExMdb将成为研究复杂疾病调控机制、推动诊断与治疗用生物标志物开发的重要资源。ExMdb数据库的访问网址为http://www.bio-server.cn/ExMdb。