Comparing transcriptional profiles of skin biopsies from healthy controls and subjects with chronic pruritus of unknown origin and atopic dermatitis

Type 2 inflammation contributes to the pathology of pruritic skin diseases such as atopic dermatitis (AD). Chronic pruritus of unknown origin (CPUO) is a skin condition of unknown etiology that typically has minimal inflammation. Yet, some patients with CPUO display signs of mildly increased type 2 inflammation. This study aims to compare skin transcriptional profiles in CPUO to healthy skin and to skin from AD, a classical type 2 inflammatory condition. Overall design: To directly compare the transcriptional profiles of healthy skin to skin from subjects with CPUO or AD, we processed skin biopsies from subjects for RNA isolation and performed RNA-sequencing.