Effects of a Uty gene trap on the transcriptomic profile of mouse cardiac left ventricles in response to castration

Previous results from the laboratory showed that the genetic make up of chromosome Y modulated the effects of castration on the transcriptome of mouse cardiac left ventricles. To test the effect of the chromosome Y Uty locus, the current study tests how castration affects the transcriptomes of cardiac left ventricles from adult male carrying a Uty gene trap (KO) in comparison to wild-type (WT)couterparts. Overall design: Frozen left ventricles were obtained from adult mice that were either UtyGt(XS0378)Wtsi (which carries a gene trap within the 4th intron of the Uty gene) or their wild-type counterparts from the isogenic strain C57BL/6.Y<129P2> adult mice. Animals had previously been either sham-operated (S) or castrated (C) at about 10 days of age.