Breast cancer cell-derived extracellular vesicles accelerate collagen fibrillogenesis and integrate into the matrix

Rheometry of collagen I hydrogels formed in the presence of extracellular vesicles, extruded plasma membrane vesicles, and synthetic liposomes; combined confocal reflectance imaging of collagen I fibrils and fluorescence confocal imaging of labelled vesicles, with processed data and analysis of collagen fibril length, matrix mesh size, and particle-fibril co-localization