GENE ASSEMBLY IN AGROBACTERIUM VIA NUCLEIC ACID TRANSFER USING RECOMBINASE TECHNOLOGY (GAANTRY)

This article examines gene assembly in Agrobacterium via nucleic acid transfer using recombinase technology (GAANTRY). It explores the molecular mechanisms underlying recombinase-mediated gene stacking and its application in plant genetic engineering. Special attention is given to the efficiency, precision, and scalability of GAANTRY systems in comparison to traditional transformation methods. The study highlights the role of site-specific recombinases in assembling multiple gene constructs into the T-DNA region of Agrobacterium tumefaciens. Furthermore, the article discusses the advantages of this technology in developing transgenic plants with complex traits, such as stress resistance and improved metabolic pathways. The findings demonstrate that GAANTRY represents a powerful and flexible tool for modern biotechnology and agricultural innovation.