Uterine histotroph and conceptus development. V. Comparative analysis of arginine-mediated transcriptomic reprogramming in conceptus trophectoderm of pigs and sheep

Arginine (Arg) is an essential functional amino acid necessary for the survival and growth of both porcine and ovine conceptus trophectoderm cells. In this study, we report transcriptome sequencing of porcine and ovine conceptus trophectoderm cells following 48 hours of incubation with or without Arg treatment. Overall design: An established porcine trophectoderm (pTr2) cell line isolated from Day 12 porcine conceptuses, as well as an established ovine trophectoderm (oTr1) cell line isolated from Day 15 ovine conceptuses were used to determine response to Arg at the physiological concentration of 0.2 mM in a 48 h culture.