Z-Stacks of microdamage formation of individual trabeculae tested in pure tensile fatigue mode

This folder contains the z-stacks of confocal images of microdamage formation in individual trabeculae, tested in pure fatigue tensile mode. The images are related to Figure 3 of the paper "Microdamage formation in individual bovine trabeculae during fatigue testing" (Frank et al., 2020). Pre-staining of initial microcracks is shown in red with Alizarin Red S and staining after mechanical testing in green with Calcein. The sample tested for 1500 cycles indicates no damage. The sample tested for 2100 cycles shows one linear microcrack. The sample tested for 3000 cycles illustrates advanced microdamage formation.

本文件夹包含单个骨小梁在纯疲劳拉伸模式下的微损伤形成z堆栈共聚焦图像。这些图像与论文《在疲劳测试过程中单个牛骨小梁的微损伤形成》（Frank 等人，2020年）中的图3相关。利用苏木精红S对初始微裂纹进行预染色，以红色呈现，机械测试后以钙黄绿素绿色染色。经过1500次循环测试的样本未发现损伤。经过2100次循环测试的样本出现一条线性微裂纹。经过3000次循环测试的样本展示了微损伤形成的晚期阶段。