Transcriptional profiling of mouse eosinophils identifies distinct gene signatures following cellular activation

Purpose: we aimed to characterized the transcriptional profile of mouse eosinophils in response to stimuli that are associated with Type 1 and Type 2 environments Methods: Eosinophils were purified from the peritoneal cavity of Il5Tg mice and stimulated with IL-4 (i.e. type 2 activated eosinophils), IFN-γ, and a combination of lipopolysaccharide (LPS, i.e. type 1 activated eosinophils) in the presence of IFN-γ. Thereafter, RNA was obtained and the transcriptome signature following each stimulation was determined using RNA sequencing. Raw sequencing reads were trimmed and filtered using fastp, then aligned to mouse genome assembly (GRCm38) using STAR 2.7.2a. Normalization and differential expression analysis were performed in R 4.0.3 using the package DESeq2. Finally, gene ontology annotations were obtained from Ensembl and pathway graphs were obtained from KEGG. Results: RNAseq analysis revealed marked differences, which were driven by the different stimulating conditions Conclusion: We demonstrate that eosinophils are polarized to distinct phenotypes following distinct stimuli. These findings contribute to the growing understanding regarding the heterogeneity of eosinophils and their possible roles in different diseases. RNAseq of eosinophils following activation with stimuli that are associated with Type 1 and Type 2 environments