Gene expression profiles under oxidative stress conditions in human HaCaT keratinocytes and the derivative cells expressing shRNA against NRF2, MLL1, or UTX.

To determine how transcriptome is altered by knockdown of NRF2, MLL1, or UTX, we performed RNA-sequencing (RNA-seq) analysis of HaCaT cells and its shRNA-expressing derivatives before and after exposure to hydrogen peroxide (H2O2) or cerium oxide nanoparticles (CeO2 NPs). Overall design: RNA-seq analysis of HaCaT cells and the derivatives following treatment with 100 µM H2O2 or 25 µg/mL CeO2 NPs for 24 hours.