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Sorting FCS files: CRISPR activation library screening with Dendra2-positive cells

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Figshare2020-04-14 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Sorting_FCS_files_CRISPR_activation_library_screening_with_Dendra2-positive_cells/11423721
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The MYC promoter-reporter system was developed with a photoconvertible fluorescent protein, Dendra2, and named “pMYC-promoter-Dendra2.” This MYC promoter-reporter system was designed to harbor a proximal MYC promoter at −3.1 kb. Both the CRISPR activation library and pMYC-promoter-Dendra2 were induced to HEK 293T cells, and Dendra2-positive cells, that are supposed that MYC should be upregulated, were collected individually by a cell sorter.
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2020-04-14
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