Exosomal miRNAs released by neural stem/progenitor cells versus astrocytes

An Affymetrix microRNA (miRNA) Array was used to compare exosomal miRNAs released by hypothalamic stem/progenitor cells (htNSC), hippocampal NSC (hippoNSC) and astrocytes in primary culture. This assay is to provide preliminary information on the general profile of exosomal miRNAs released by these cells, while to study any particular miRNA species should require further quantitative analyses such as qPCR based on enough sample sizes. Exosomal RNAs were obtained from the culture media of htNSC, hippoNSC and astrocytes, adjusted to the same amount and run through an Affymetrix miRNA Array chip.