Single-cell characterization of human conjunctiva tissue, organoids and air-liquid interface cultures (10X)

We mapped the cell types present in human conjunctiva tissue, organoids and air-liquid interface cultures exposed or not to IL4/13. Overall design: The following samples were subjected to single-cell mRNA sequencing: (1) human conjunctival organoids cultured in expansion medium for 5 days (donor 16 – palpebral – passage 5), (2) human conjunctival organoids cultured in expansion medium for 10 days and in differentiation medium for 7 days (donor 16 – palpebral – passage 4), (3) human conjunctival ALI when cells had just reached confluence (i.e. 3 days after seeding, named ALI day 0, from donor 16 – palpebral – passage 6), (4) human conjunctival ALI 3 days after shifting to air-liquid interface (donor 16 – palpebral – passage 5), (5) human conjunctival ALI 17 days after shifting to air-liquid interface (donor 16 – palpebral – passage 3, donor 4 – bulbar – passage 10), (6) human conjunctival ALI 15 days after shifting to air-liquid interface exposed to IL4/13 (donor 4 – bulbar – passage 4), and (7) human tissue from 4 pooled donors (all bulbar conjunctiva).