Identification of a novel antitumor CD4+ T cell cluster orchestrating antitumor immunity

scRNAseq-based unsupervised clustering and clonotyping revealed that Th1/17 and CCR6 SP clusters formed metacluster Th7R, which was distinct from Th1 or Th17, characterized by high expression of the IL-7 receptor. Use of this cluster to assess antitumor CD4+ T cell immunity from the peripheral blood and to predict the efficacy of immune checkpoint inhibitors will pave the way for novel antitumor immunotherapy strategies for patients. Investigating how differences in gene expression and TCR repertoires of T cells in blood affect the response of pembrolizumab therapy. All samples in this dataset were obtained from patients before receiving pembrolizumab therapy. GEX and TCR samples with the same P* identifier were obtained from the same set of cells.